NR4A2/3 regulates neutrophil number in zebrafish larvae. The mpx:GFP zebrafish larvae were injected with (A-B, D, F) NR4A2 (0.5 pM, 1 pM, or 2 pM), (A, C, E, G) NR4A3 (0.125 pM, 0.25 pM, or 0.5 pM), (A-G) or control MO (2 pM) at the 1-cell stage. (A) Neutrophils were visualized as GFP-positive events by fluorescent microscopy. (B-C) Total neutrophil numbers were assessed at 80 hpf (n = 20, performed as 3 separate experiments). Larvae were injured at 72 hpf, and neutrophil counts were performed at (D-E) 6 and (F-G) 24 hours postinjury (n = 16, performed as 2 separate experiments). Statistical analysis was performed by ANOVA with Dunnett’s posttest. Significant differences compared with control MO were denoted by ***P < .001 and ****P < .0001.