Hemostasis parameters in non-FVL carriers and FVL carriers. (A) Plasma concentrations of APC, F1+2, TAT, and D-dimer were measured in FVL carriers without a history of thrombosis (n = 22, thereof 8 homozygous), and in non-FVL carriers (n = 81). Data are presented as median and interquartile range (IQR). The Mann-Whitney test was used to calculate significance; P values <.05 are shown. Plasma levels of FVIIa (B), F1+2 (C), TAT (D), and APC (E) were measured before (t = 0) and after IV injection of 15 µg/kg rFVIIa in 12 heterozygous FVL carriers without a history of thrombosis (●, solid lines) and in 12 non-FVL carriers (○, intersected lines). Red data points and connections indicate a significant increase (P < .05) compared with baseline (t = 0). P values were calculated using the paired Student t test or the Wilcoxon signed-rank test, depending on the normality of data. The Bonferroni method was applied to correct for multiple testing. Plasma levels of TAT in 3 homozygous FVL carriers are additionally shown (gray circles, dotted lines). (F) Area under the APC generation curve (AUC).