Hdac8 modulates stress-induced p53 acetylation and target gene expression. (A) Coimmunoprecipitation (IP; anti-p53) and immunoblotting (anti-HDAC8 or anti-p53) analyses in 32D cells before or 2 to 6 hours after exposure to radiation (3 Gy). (B) Western blot analysis of acetylated p53, total p53, and β-actin before and after (3-6 hours) after irradiation (IR; 3 Gy) in control (Ctrl) or Hdac8^Δ/Δ BM (2-3 months old) cells. (C) Relative expression of Bax, Noxa, Puma, and Cdnk1a, without IR and 6 hours after IR (3 Gy) in Ctrl, Hdac8^Δ/Δ, Hdac8^Δ/Δ/p53ER^KI/KI, or p53ER^KI/KI BM cells, measured by qRT-PCR assays. Bars represent mean ± standard deviation (n = 3-5). (D) Relative expression of p53 targets Bax, Noxa, and Puma in WT BM cells treated with HDAC8-selective inhibitor 22d (10 μM for 2 hours) or vehicle, without IR or 6 hours after IR (3 Gy). *P < .05, **P < .01, ***P < .001.