Complete loss of F10 results in progressive adult lethality. (A) Genotype distributions of offspring from separate f10^+/− incrosses evaluated at various stages demonstrate loss of homozygous mutants after 25 dpf. (B) Survival curves of closely monitored clutches of zebrafish offspring from f10^+/− incrosses demonstrate progressive loss of 75% of homozygotes by 50 dpf and 100% by 115 dpf. There was no significant loss of heterozygotes (P > .05 by log-rank testing). Larvae were genotyped at 3 dpf and selected individuals were observed daily. There was ∼20% background loss of individual fish across all genotypes up to 20 dpf, which is typical during wild-type fish development. (C) Citrated plasmas from 1-month-old fish were recalcified and incubated with human fibrinogen for 90 minutes and absorbance (405 nm) was measured every 2 minutes. Data shown are the average of 2 experiments (n = 5 total pairs of fish for each genotype). The average time to half-maximal absorbance and maximum absorbance were calculated for each genotype and data were analyzed by using the Student t test. Bovine thrombin was used as a positive control. Max, maximum; min, minutes.