Figure 2.
Figure 2. Genome editing of f10 using TALENs results in a frameshift and null allele. (A) Schematic diagram of TALENs used for targeted mutagenesis of f10. (B) Targeting of f10 exon 5 using a TALEN resulted in frameshift mutations. The 17-bp deletion mutant used for subsequent studies is shown. (C) Expression of f10 mRNA is reduced in heterozygous and undetectable in homozygous mutants, as evaluated by RT-PCR (each genotype was evaluated in triplicate). (D) Whole-mount in situ hybridization with an antisense probe demonstrates absence of expression in f10−/− mutants. Anterior is toward the left, and dorsal is toward the top. Scale bar, 100 µm. Chr1, chromosome 1; C-term, C-terminal domain; DBD, DNA-binding domain; Fok I, Fok I nuclease; NLS, nuclear localization signal; N-term, N-terminal domain.

Genome editing of f10 using TALENs results in a frameshift and null allele. (A) Schematic diagram of TALENs used for targeted mutagenesis of f10. (B) Targeting of f10 exon 5 using a TALEN resulted in frameshift mutations. The 17-bp deletion mutant used for subsequent studies is shown. (C) Expression of f10 mRNA is reduced in heterozygous and undetectable in homozygous mutants, as evaluated by RT-PCR (each genotype was evaluated in triplicate). (D) Whole-mount in situ hybridization with an antisense probe demonstrates absence of expression in f10−/− mutants. Anterior is toward the left, and dorsal is toward the top. Scale bar, 100 µm. Chr1, chromosome 1; C-term, C-terminal domain; DBD, DNA-binding domain; Fok I, Fok I nuclease; NLS, nuclear localization signal; N-term, N-terminal domain.

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