Generation of transgenic mice (Tg-sP-selectin) expressing chronically elevated plasma levels of sP-selectin. (A) Schematic of the sP-selectin transgene. (B) Plasma levels of total sP-selectin (measured with anti-P-selectin IgG) or transgenic sP-selectin (measured with mAb HPC4) in WT or Tg-sP-selectin mice. The data represent the mean ± SEM from 8 experiments in each group. (C) Western blots (WB) of P-selectin from platelet lysates or from immunoprecipitates of plasma from mice of the indicated genotype, or of recombinant sP-selectin (right lane). The data are representative of 5 experiments.