No significant differences in B- or myeloid cell populations in the adult bone marrow of Tr4^+/−mice. (A,C) Representative flow cytometric diagram of total bone marrow cells recovered from 6- to 8-week-old WT (closed circles) or Tr4 heterozygous mutant (open circles) mice. RBCs were lysed and then the remaining bone marrow cells were stained with (A) α-B220 and α-CD19 for B-cell analysis or with (C) α-CD11b and α-Gr1 for myeloid cell analysis. The total number of cells per femur was quantified for the gates indicated in panels A and C. (B,D). No significant differences were observed by Student t test comparing Tr4 heterozygous mutant with WT bone marrow cells.