A diagram of a CAR is shown. The antigen-binding domain of a CAR is attached to intracellular T-cell signaling moieties by an extracellular hinge domain and a transmembrane region. The CAR antigen-binding domain is usually a scFv derived from a monoclonal antibody. Examples of costimulatory domains are CD28 and 4-1BB. The T-cell activation domain is usually from the CD3Zeta molecule. Professional illustration by Patrick Lane, ScEYEnce Studios.