Figure 1.
![Figure 1. Two MK populations with distinct granularity are present in the in vitro human stem cell–derived MK culture. (A) Representative size (forward-scatter area [FSC-A]) and granularity (side-scatter area [SSC-A]) profiles of CD42a+ MKs at specified time points during the differentiation of human CD34+ hematopoietic progenitors. LG and HG MKs are gated as shown. (B) Day 14 LG CD42a+ MKs were sorted and cultured for another 6 days (top). Size and granularity of MKs 3 days and 6 days postsort are shown. Day 14 HG CD42a+ MKs were similarly sorted and cultured (bottom). Representative studies of 3 independent studies.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/130/2/10.1182_blood-2017-01-761049/4/blood761049f1.jpeg?Expires=1722561352&Signature=KPlBXEcHVeL7xSKRocneLHGpxa4hl7U3Qn~pQ5mYNeMYryGbf7dYpb6l~9-qFJmWl9Q9FVG4GvcskWa4y78hVYRGK7o0w08QE5i300uVMGULNwQriPpOuhVQYsatcKomDExAlaL7c6uASXN-Ff0M3~MrN9DO~-JtYTWEcL9V-tbu7Btgw~iUsgS5w3YpNUy2UIJips0siQwn4BJKWjHgjS7Z4k9ULCYc9Ji56~ZnzB-l8f2tPFHumvuOZ3gXDYEkXzJAIYSDwLIR4y1v88A9lSSg066HPkTk-l31lOp5pvokNW2MkYEdl7GGCFbyld8JXdDH9EMJwSME6II0ex3xKA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Two MK populations with distinct granularity are present in the in vitro human stem cell–derived MK culture. (A) Representative size (forward-scatter area [FSC-A]) and granularity (side-scatter area [SSC-A]) profiles of CD42a+ MKs at specified time points during the differentiation of human CD34+ hematopoietic progenitors. LG and HG MKs are gated as shown. (B) Day 14 LG CD42a+ MKs were sorted and cultured for another 6 days (top). Size and granularity of MKs 3 days and 6 days postsort are shown. Day 14 HG CD42a+ MKs were similarly sorted and cultured (bottom). Representative studies of 3 independent studies.
