In vitro differentiation potential of monocytes and myeloid DCs. (A) Sorted CD14⁺ monocytes, CD16⁺ monocytes, and CD1c⁺ myeloid DCs from healthy controls cultured for 3 days in conditions as shown. Langerin^highCD1a⁺ gates depicted contain LCH-like cells with Birbeck granules. Notch ligands DLL1 And DLL4 were presented on transfected mouse OP9 cells. Representative data of 5 independent experiments. (B) Sorted CD14⁺ monocytes, CD16⁺ monocytes, and CD1c⁺ myeloid DCs from healthy controls cultured for 7 days with M-CSF and 5% human serum (HS) to reveal potential to differentiate into foamy macrophages. Representative phase contrast images of 5 independent experiments.