Figure 4.
Mononuclear profile and BRAF allele frequency in CD34+ BM progenitors. (A) Relative proportions of progenitor fractions among CD34+ BM mononuclear cells compared with healthy controls (n = 21), expressed as percentage of total live cells. Error bars depict 95% confidence intervals of healthy controls. Populations: B/NK, B/NK cell progenitors; LMPP, lymphoid-primed multipotent progenitors; MEP, megakaryocyte–erythroid progenitor; MPP, multipotent progenitor. (B) The distribution of BRAFV600E among CD34+ BM progenitors. Gray shading indicates positive fractions. BM aspirate was obtained in parallel with the peripheral blood shown previously. *Child; †NRASQ61R Sanger sequencing. NT, not tested owing to insufficient number of cells.
