Chd7 deficiency delays leukemia initiation induced by Cbfb-MYH11. (A-C) The indicated groups of mice were treated with ENU to induce additional mutations and with poly(I:C) to induce the expression of Cbfb-MYH11 and/or Chd7 deficiency. Three weeks after poly(I:C) treatment, mice were euthanized and flow cytometry assays were performed. (A) Leukemia development monitored by measuring the c-Kit⁺/Sca1^– population in peripheral blood (PB). (B) Representative FACS plots of bone marrow cells gated on single cells (upper plots), Lin^– cells (middle plots), and LK cells (bottom plots). (C) Bar graph showing the percentages (mean ± standard error of the mean [SEM]) of Lin^–, LK, LSK, AMP, CMP, GMP, and MEP compartments in the bone marrow of mice of the indicated genotypes. *P < .05; **P < .01; ***P < .001; ****P < .0001, each compared with the control group except for (#), for which the comparisons were between the Chd7^f/fMx1-CreCbfb^+/56M group and the Mx1-CreCbfb^+/56M group.