Super-enhancers at the known cancer genes. (A-C) The ChIP-seq gene tracks represent H3K27Ac signals at the PIK3R1 (A), TP73 (B), and CCR4 (C) gene loci. See the legend to Figure 1D for details. (D) CCR4 mRNA expression in 3 T-ALL cell lines (Jurkat, KOPT-K1, and DND41), 4 ATL cell lines (TL-Om1, ATL-55T[–], MT-2, and ATL-43b[−]), and 10 primary ATL samples was measured by qRT-PCR analysis. The expression values were normalized to β-actin expression, and the data are presented as the fold change compared with TL-Om1 cells (mean ± standard deviation of duplicates). (E) Schematic depicting the positions and types of somatic alterations in the CCR4 gene. The mRNA sequences of the coding region were individually analyzed by qRT-PCR followed by Sanger sequencing. The x-axis indicates the amino acid (aa) position, and the y-axis indicates the number of mutations. TM, transmembrane domain.