Super-enhancer profiling in ATL samples. (A) Enhancers were ranked by increasing H3K27Ac signal in 10 primary ATL cases and 1 ATL cell line (TL-Om1). The number of super-enhancers (SEs) is shown for each sample. Examples of genes that were commonly associated with super-enhancers in ≥5 primary ATL cases are shown. (B) PCA was performed for 1 T-ALL cell line (Jurkat), 1 normal thymus sample, 3 normal CD4⁺ T-cell populations (Th1, Th2, and Th17), 1 ATL cell line (TL-Om1), and 10 primary ATL cases (ATL1-10) based on the super-enhancers identified in each sample. Each circle represents a sample, and each color represents the type of sample. (C) Genes commonly associated with super-enhancers in primary ATL cases were subjected to gene ontology analysis (biological process) and KEGG pathway analysis. The top 10 categories are shown with combined scores. (D-E) The ChIP-seq gene tracks represent the H3K27Ac signal in immature T cells (Jurkat and normal thymus), normal CD4⁺ T-cells (Th1, Th2, and Th17), and ATL cells (TL-Om1 and 10 primary ATL samples) at the CD2 (D) and IL2RA/CD25 (E) gene loci. The x-axis indicates the linear sequence of genomic DNA, and the y-axis indicates the total number of mapped reads per million. The black boxes in the gene map represent exons, and the arrows indicate the location and direction of the TSS. The super-enhancers are shown as red lines.