ZFP521 regulates murine HSC self-renewal and differentiation. (A) Schematic representation of serial competitive transplantation studies. (B-D) Peripheral blood analysis of primary transplant recipients (10 recipients per genotype) of WT (Zfp521^+/+) and Zfp521 KO (Zfp521^−/−) whole fetal liver cells showing (B) total donor chimerism; (C) donor contribution to myeloid, B, and T cells; and (D) granulocyte chimerism. (E-H) Bone marrow analysis at 16 weeks posttransplantation showing (E) total chimerism and frequencies of (F) HSCs, MPPs (MPP1 and MPP2), (G) CMPs, GMPs, megakaryocyte-erythroid progenitors (MEPs), and (H) CLPs. Peripheral blood analysis of secondary transplant recipients (10 recipients per genotype) showing (I) total donor chimerism; (J) donor contribution to myeloid, B, and T cells; and (K) granulocyte chimerism. (L-P) Bone marrow analysis at 16 weeks posttransplantation showing (L) total chimerism and frequencies of (M) HSCs, MPP1, and MPP2; (N) CMPs, GMPs, and MEPs, (O) megakaryocyte progenitors, and erythroid progenitors, and (P) CLPs. Unpaired t test: *P < .05, **P < .01, ***P < .001. BM, bone marrow; EP, erythroid progenitor; Gr, granulocyte; MkP, megakaryocyte progenitor; PB, peripheral blood; wBM, whole bone marrow.