Ask1 regulates in vivo thrombosis. (A) Percentage of adult mice testing positive for fecal occult blood. (B) Tail-bleeding times performed by amputating the terminal 3-mm segment of the tail of anesthetized 4- to 6-week-old WT (n = 17) and Ask1−/− (n = 17) mice and immersing them into warm saline solution prior to genotyping. Bleeding time was measured as the time from the start of bleeding to cessation of bleeding. The bleeding was manually stopped for those for which bleeding continued for more than 10 minutes. (C) Tracings of Doppler flow of carotid artery after 10% FeCl3-induced injury in anesthetized 8- to 12-week-old WT (n = 13) and Ask1−/− (n = 14) mouse right common carotid artery. Duration of the injury is denoted by 1; time to occlusion is denoted by 2; cessation of flow is denoted by 3. (D) Quantitation of occlusion time from panel C. (E) Quantitation of percentage of mice from panel C with stable or unstable occlusion or no occlusion. *P < .05; **P < .01; ***P < .001.
