RUNX1 is required for intracellular NOTCH1 binding and for chromatin accessibility at the N-Me. (A) H3K27ac, NOTCH1, and RUNX1 enrichment at the human MYC super-enhancer is shown in genome browser tracks (genome.ucsc.edu, human hg19). (B) The mouse genomic region (mm10) encompassing Myc and its enhancer loci located 1.27 Mb from the TSS are shown. The RBPJ- and RUNX-binding sites are depicted. (C) Recruitment of RUNX1, intracellular NOTCH1, H3K27ac, and H3 to the mouse Myc enhancer was determined by ChIP-qPCR in control or Runx1-deleted mouse T-ALLs. (D) The degree of open chromatin at the N-Me enhancer region in control or Runx1-deleted mouse T-ALLs was determined by ATAC-quantitative PCR. Data are the mean of 3 or 4 independent experiments and error bars represent ± SEM. *P < .05; **P < .005; ***P < .0005, two-way ANOVA multiple comparisons test.