RUNX1 regulates a subset of TAL1- and NOTCH1-regulated genes. (A) mRNA was isolated from mouse T-ALL cells 48 hours after vehicle or 4-OHT treatment, and the expression of a subset of a RUNX1- and TAL1-regulated genes was determined by qRT-PCR. Three to 4 independent experiments were performed, and data are shown as means ± standard errors of the means (SEM). (B) Gene set enrichment analysis of RUNX1-regulated genes and genes changed on reactivation of NOTCH1 by γ-secretase inhibitor (GSI) washout (51). RUNX1 target genes that were significantly downregulated by RUNX1 knockdown in Jurkat cells were used as a data set (22). (C) The expression of a subset of NOTCH1-regulated genes in Runx1-deleted mouse T-ALL cells was determined by qRT-PCR. Three to 4 independent experiments were performed and data are shown as means ± SEM. *P < .05; **P < .005; ***P < .0005, Student t test.