Figure 4.
![Figure 4. Intracellular cytokine staining of CD8+ DILs after restimulation with mature DCs alone (mDC) or WT1 mRNA-electroporated DCs (mDC WT1). The WT1-specific T-cell cytokine response was evaluated by comparing mDC WT1 with mDC (all patients examined: UPN03, 05, 06, 08, 14, 17, 21, 22, 29, 30, 34, 35, and 47; long-term [LT] survivors: UPN06, 08, 14, 17, 21, 22, 29, 34, and 35; non-LT survivors: UPN03, 05, 30, and 47). IFN-γ+, interleukin-5+ (IL-5+), or TNF-α+ CD8+ T cells are shown for all patients (A), LT survivors (B), and non-LT survivors (C). Polyfunctional TNF-α+/IFN-γ+ CD8+ T cells are shown in the same patients (D).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/130/15/10.1182_blood-2017-04-780155/4/blood780155f4.jpeg?Expires=1724927366&Signature=HX5VG-4HZPXL5jiuPTXfViUld6lfcwJhS3H56HIJn3T6M0IYijFKzLY88BvoiEhxDAd8LjqYXkW6CWq0pwXnP5nzZSH83I77HYuejS~JRkcDG1Rrzdd2p~F6VakacodX5qgHMzNu9~HofNBuZ4BJ1F90JNvuMEkekzpY~-u7KA8mM1KMk8-Bqql2t~BguO0gyMJfZn75stVozQJMIbdthsTigwAmbSaTpgtn4xtXwUQjV1CG4KZteNndk7GBTihKhP48FdNgcKkK0fYxNPXz7cV1F9t7C2kJJpoVNjhTg0wOSguNH2dkfqbZWEoKl0TIuN1vr8vNulRDSLGSfmxVWA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Intracellular cytokine staining of CD8+DILs after restimulation with mature DCs alone (mDC) or WT1 mRNA-electroporated DCs (mDC WT1). The WT1-specific T-cell cytokine response was evaluated by comparing mDC WT1 with mDC (all patients examined: UPN03, 05, 06, 08, 14, 17, 21, 22, 29, 30, 34, 35, and 47; long-term [LT] survivors: UPN06, 08, 14, 17, 21, 22, 29, 34, and 35; non-LT survivors: UPN03, 05, 30, and 47). IFN-γ+, interleukin-5+ (IL-5+), or TNF-α+ CD8+ T cells are shown for all patients (A), LT survivors (B), and non-LT survivors (C). Polyfunctional TNF-α+/IFN-γ+ CD8+ T cells are shown in the same patients (D).
