Human AML acquire mouse mitochondria in NSG xenograft model. (A) Schematic representation of patient-derived xenograft model used for these experiments. (B) Into NSG mice, 2 × 106 primary AML cells (4 individual patients with AML) were injected intravenously. Engraftment was measured using human CD33 and human CD45. In the dot plot, each AML engraftment into NSG mice is shown for bone marrow and spleen. (C) Engrafted AML were purified from the mouse BM using human CD45 cell sorting. Shown in the flow figure are unsorted and sorted AML populations from the xenograft. (D) Total DNA was extracted from the purified AML and analyzed by PCR for murine and human specific mitochondrial and genomic DNA. PCR products were visualized by agarose gel electrophoresis. (E) OCI-AML3 cells engrafted into NSG mice were also analyzed by PCR and agarose gel electrophoresis.
