Activated PC rescues defective insulin signaling in DKD. (A-D) Schematic illustration of interventional studies in mice with STZ-induced hyperglycemia (A, top). Dot plot summarizing albuminuria (A, bottom); representative images and bar graph of the glomerular filtration barrier (B, upper panel; C, transmission electron microscopy; GBM, glomerular basement membrane, arrows; scale bar: 2 µm). Representative images and bar graph showing extracellular matrix deposition (B, lower panel; D, PAS staining; scale bar: 20 µm). (E-H) Representative immunoblots (E) and bar graphs (F-H) showing nuclear levels of ER transcription factors in renal cortex samples. C, control mice without diabetes; DM, diabetic mice. Mean ± SEM of at least 15 (A), 10 (C-D), or 6 (F-H) mice per group. *P < .05; **P < .01 (C-D, F-H: ANOVA).