ROS-mediated inhibition of cytokine production imparts a selective role for STIM2 in neutrophils. (A) Analysis of TNFα production in WT neutrophils treated with the NADPH oxidase inhibitor DPI. Bone marrow cells (1 × 10⁶) from WT mice were incubated with zymosan (100 μg/mL) for 5 hours with or without DPI (10 μM), and TNFα levels were measured by intracellular staining. (B) Analysis of NF-κB activation in WT neutrophils treated with DPI. Isolated neutrophils were stimulated with zymosan (100 μg/mL) in the presence or absence of DPI (10 μM), and nuclear and cytoplasmic fractions were blotted for the indicated proteins. (C) Bone marrow cells (1 × 10⁶) from WT, Stim1^VavCre, Stim2^VavCre, and Stim1/2^VavCre mice were incubated with zymosan (100 μg/mL) for 5 hours with or without the NADPH oxidase inhibitor DPI (10 μM) or PMA/Ionomycin, and TNFα levels were measured by intracellular staining. (C) Representative FACS plots. (D-E) Mean ± SEM compiled from 2 to 3 independent experiments. *P < .05.