STIM2 is minimally required for neutrophil bactericidal responses. (A-C) Analysis of ROS production in WT, Stim1^VavCre (A), and Stim2^VavCre neutrophils (B) stimulated with formyl-methionine-leucine-phenylalanine (fMLF) or zymosan. (C-D) Analysis of phagocytosis by WT, Stim1^VavCre, Stim2^VavCre, and Stim1/2^VavCre neutrophils. Neutrophils were incubated for 30 minutes with pHrodo Red-labeled S aureus opsonized with serum or anti-S aureus immunoglobulin G or pHrodo Red zymosan particles. Internalized particles were quantified by percentage of phycoerythrin-stained cells, compared with samples on ice (D). A representative FACS plot is shown in (C). Mean ± SEM compiled from 2 to 4 independent experiments. *P < .05.