SOCE is not required for neutrophil development. (A-B) Expression of Stim1 and Stim2 determined by qPCR on cDNA from WT neutrophils either resting (A) or stimulated with zymosan (100 μg/mL) for 4 hours (B). (C) STIM protein levels in neutrophil lysates from Stim1^f/f, Stim1^VavCre, Stim2^VavCre, and Stim1/2^VavCre mice blotted for STIM1, STIM2, or actin control. (Right) Quantitation of band intensities from 2 to 3 blots. (D-F) Analysis of neutrophil number and phenotype in bone marrow isolated from Stim1^f/f, Stim1^VavCre, Stim2^VavCre, and Stim1/2^VavCre mice. Bar graphs represent mean ± standard error of the mean (SEM) compiled from 2 to 3 independent experiments. (D) Percentage of Ly6G^hi, CD11b⁺ neutrophils in bone marrow (n = 9-11). (E) Representative FACS plots from D. (F) Surface levels of CD16/32 and Ly6G (markers of mature neutrophils).