Inhibition of PIM kinases in RS cells increases T-cell activation. (A) SUP-HD1 and HDLM-2 RS cells were treated for 120 hours with DMSO alone or B489 (2 µM). Normal blood-derived naive T cells were stimulated with human T-activator CD3/CD28 Dynabeads and mixed with pretreated RS cells at 1:4 ratio (T cells to RS cells). After 16 hours of coculture, CD69 and CD25 expression on T-cell surface was measured by flow cytometry. (B) Relative changes in T- cell activation, presented as changes in CD69⁺/CD25⁻ (Q1 in Figure 7A), CD69⁺/CD25⁺ (Q2 in Figure 7A), CD69⁻/CD25⁻ (Q3 in Figure 7A), and CD69⁻/CD25⁺ (Q4 in Figure 7A) staining in T cell, averaged from 3 independent experiments. P values were determined using the 2-sided Gosset’s t-test: ****P < .0001.