EI-tPA reduces the toxicity of LPS in wild-type mice. (A) Kaplan-Meier survival curves are shown for wild-type C57BL/6J mice treated by IV injection with 50 µg h-EI-tPA (n = 6; green line) or vehicle (n = 6; blue line), 1 h after IP injection of LPS at 1.5 times the LD₅₀. Significance was determined by log-rank test. (B) ELISA was performed to detect endogenous tPA in mouse plasma harvested from mLRP1^+/+ and mLRP1^−/− mice, 3 h after IP injection of LPS at 0.5 times the LD₅₀ or vehicle. Each data point represents a duplicate analysis of a plasma sample from a different mouse (mean ± SEM; 1-way ANOVA with Tukey’s post hoc analysis). **P < .01.