Profiling protein phosphorylation in tPA-treated BMDMs. (A) BMDMs were isolated from WT C57BL/6J mice and treated with h-EI-tPA (12 nM) or vehicle for 1 h. Protein phosphorylation was analyzed by array. (B) Changes in protein phosphorylation associated with h-EI-tPA treatment in comparison with cells treated with vehicle. Each of the analyzed phoshoproteins is demarcated with a box in panel A. (C) BMDMs were treated with increasing concentrations of h-EI-tPA (0.1-25 nM) for 1 h. Cell extracts were subjected to immunoblot analysis to detect phospho-ERK1/2 and total ERK1/2.