Chromatin accessibility is reduced at hypermethylated sites. (A) Chromatin accessibility measured by DNase-seq across hypermethylated TSS proximal and TSS distal DMCs in CD34⁺ cells (blue) and the AML cell lines HL-60 and NB4 (red). (B) Chromatin accessibility across hypermethylated sites in CD34⁺ cells (blue) and CN-AML patients (red). (C) An example of a silenced DHS in the WT1 gene body, which is accessible in CD34⁺ cells (blue) and inaccessible in HL-60, NB4, and CN-AML patients (red). RPM, reads per million.