Zeb2 deletion changes the gene expression profile. (A) A heat map of at least 2 fold down- or upregulated genes. Clustered heat map visualizes normalized expression values of genes that showed an at least 2-fold up- or downregulation together with a nominal P value < 1%. The RNA was isolated from 3 pairs of independently sorted control and Zeb2^Δ/ΔMx1-Cre HSC (LKS-SLAM) at 8 weeks after Poly(I:C) administration. (B) Selected KEGG pathways with significant alterations after Zeb2 deletion are shown here (the complete list of pathways analyses is included as supplemental Table 5). (C-E) Representative western blots showing phosphorylation of indicated signaling proteins in total lysates of BM cells (C), lineage-negative cells (D), and Mac1⁺ myeloid cells (E) from Zeb2^Δ/ΔMx1-Cre or control mice in the presence of 10 μg/μL G-CSF, IL-3, or IL-6 for 15 minutes. Data shown as a representative plot from at least 3 independently isolated biological replicates. Means ± SEM is shown. (F) Flow cytometric analysis of phosphorylated ERK1/2 in LKS cells treated with the stem cell factor (SCF) of Zeb2^Δ/ΔMx1-Cre and control mice revealed reduced signal transduction in absence of Zeb2. (G) Representative FACS plot of phospho-STAT3 expression in prepro-B cells after stimulation with IL-7 shows severely reduced signaling in Zeb2^Δ/ΔMx1-Cre prepro-B cells.