The frequencies of iNKT cells in blood and spleen are decreased in patients with HHV-8 MCD. PBMCs and SMCs from controls (HDs and patients with KS) or patients with HHV-8 MCD were stained ex vivo for surface expression of CD3, iTCR mAbs, CD4, and CD8. (A) Representative flow cytometry plot and cumulative data showing frequencies and median of iNKT cells in the PBMCs of 29 HDs, 21 KS patients, and 25 patients with HHV-8 MCD (17 HIV⁺ and 8 HIV⁻). Significant P < .05, Mann-Whitney test. (B) Representative flow cytometry plot and cumulative data showing frequencies and median of iNKT cells in the SMCs of 8 HDs and 7 patients with HHV-8 MCD. Significant P < .05, Mann-Whitney test. (C) Cumulative data showing frequencies and median of iNKT cell subsets (ie, CD4⁺, CD8⁺, CD4⁻CD8⁻ (DN), and CD4⁺CD8⁺ (double positive [DP]) in the PBMCs of 27 HDs, 6 patients with HHV-8 MCD HIV⁻, and 10 patients with HHV-8 MCD HIV⁺. Significant P < .05, Mann-Whitney test.