FOXO1 gene signature is overrepresented in AE preleukemia and leukemia cells. (A) RNA-seq analysis was performed on human CD34⁺ HSPCs transduced with indicated genes. Heat map depicting hierarchical clustering of 1000 significantly differentially expressed genes of AE, FOXO1 WT, and FOXO1 DB cells compared with MIG cells. The detailed gene list can be found in supplemental Table 1. (B) Pathway enrichment analysis of FOXO1-activating target genes showed enrichment of AE and HSC signatures. (C) Heat map showing the expression level of FOXO1-activating gene signatures in different subtypes of AML patient samples. The data set was from Ross et al.²²  (D) Bean plot depicting the average of the mean-centered log₂ expression values of FOXO1-activating genes in different subtypes of AML patient samples. The data set was from Ross et al.²²  Black lines show the means of subgroups; each white line represents the value of individual samples; polygons represent the estimated density of the data. P values were calculated by 2-tailed Student t test.