![Eight-week-old Bmp6fl/fl;Tek-Cre+ mice exhibit liver Bmp6 and Hamp deficiency and serum iron overload, whereas Bmp6fl/fl;Alb-Cre+ and Bmp6fl/fl;LysM-Cre+ mice do not. Eight-week-old littermate male (left) and female (right) Bmp6+/+, Bmp6+/−, and Bmp6−/− global knockout mice (red bars) and Bmp6 conditional knockout mice in ECs (Bmp6fl/fl;Tek-Cre+ [blue bars]), macrophages (Bmp6fl/fl;LysM-Cre+ [green bars]), and hepatocytes (Bmp6fl/fl;Alb-Cre+ [purple bars]) compared with littermate controls (Bmp6fl/fl;Cre–) were analyzed for (A) total liver hepcidin (Hamp) and (D) Bmp6 relative to Rpl19 mRNA by qRT-PCR, (B) serum iron, and (C) transferrin (Tf) saturation. n = 4-11 mice per sex per group (supplemental Table 2). Results are reported as mean ± SEM of –ΔCt as in Figure 1. Bmp6 mRNA levels for Bmp6+/+ and Bmp6−/− mice, stratified by sex, were replotted from Figure 1 for comparison. *P < .05, **P < .01, ***P < .001 relative to control Bmp6+/+ mice or Bmp6fl/fl;Cre– mice by one-way ANOVA with Dunnett’s post hoc test or Student t test. nd, not detectable.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/129/4/10.1182_blood-2016-06-721571/4/blood721571f3.jpeg?Expires=1720285266&Signature=YrkSn63up5R0CsprFdioLqwoWhAg8gksW07Gn83C1I3G055GB5DiSiv2U~2UG2lHi8UZIc6I3u2Dft9vz1OYzWEJj36VI~KNW3ZjpVeENfgAHSvgOk4kdUur9zw4PXP3MLFmYZWVWECn7dKOA~31dr5gM3i2X3dyhhHpVzhNnB3y8MHeBUS7yMKaJH3UsSIfaPV6aNP72iGXzXs14QgzDpkDxg96VvYfDbH90Mfh9FfZ1LakqacgjUzmvh9epIekRQEKMzfqsMedU1H57QLsuYGj8bxruQEO-h6APOmKqMDJoAKfXO6r7oQ8cF7dvGtJ6phPs8rF8tLewZ1pg4SYcQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Eight-week-old Bmp6fl/fl;Tek-Cre+ mice exhibit liver Bmp6 and Hamp deficiency and serum iron overload, whereas Bmp6fl/fl;Alb-Cre+ and Bmp6fl/fl;LysM-Cre+ mice do not. Eight-week-old littermate male (left) and female (right) Bmp6+/+, Bmp6+/−, and Bmp6−/− global knockout mice (red bars) and Bmp6 conditional knockout mice in ECs (Bmp6fl/fl;Tek-Cre+ [blue bars]), macrophages (Bmp6fl/fl;LysM-Cre+ [green bars]), and hepatocytes (Bmp6fl/fl;Alb-Cre+ [purple bars]) compared with littermate controls (Bmp6fl/fl;Cre–) were analyzed for (A) total liver hepcidin (Hamp) and (D) Bmp6 relative to Rpl19 mRNA by qRT-PCR, (B) serum iron, and (C) transferrin (Tf) saturation. n = 4-11 mice per sex per group (supplemental Table 2). Results are reported as mean ± SEM of –ΔCt as in Figure 1. Bmp6 mRNA levels for Bmp6+/+ and Bmp6−/− mice, stratified by sex, were replotted from Figure 1 for comparison. *P < .05, **P < .01, ***P < .001 relative to control Bmp6+/+ mice or Bmp6fl/fl;Cre– mice by one-way ANOVA with Dunnett’s post hoc test or Student t test. nd, not detectable.
