ZEB2 is a differentiation switch in normal hematopoiesis and leukemogenesis. (A) Cartoon illustration of Zeb2 “ON” and “OFF” expression switches (depicted as green and red, respectively), indicating that with Zeb2 expression, all hematopoietic stem, progenitor, and mature lineages are produced at normal numbers (++). However, on deletion of Zeb2 expression, multilineage differentiation and progenitor production are perturbed. Red arrows highlight increased HSC, MEP, and granulocytes in the absence of Zeb2. (B, left) Illustration of ZEB2 expressing AML cells undergoing proliferation and self-renewal. (B, right) ZEB2 shRNA-mediated knockdown results in differentiation and decreased proliferation of AML cells. B, B-cell; Er, erythrocyte; GMP, granulocyte-monocyte progenitor; Gr, granulocyte; MEP, megakaryocyte-erythrocyte progenitor; Mk, mature megakaryocyte; Mo, monocyte.