Figure 3.
Figure 3. Effect of P2Y12 gene knockdown in murine ovarian cancer cells on the growth of orthotopic ovarian tumors. (A) Expression of P2Y12 mRNA in control and mP2Y12 CRISPR-Cas9 ID8-VEGF cells before the injection into mice (n = 3 triplicate experiments). (B) Representative images of necropsy in mice carrying control or mP2Y12 CRISPR-Cas9 ID8-VEGF cell–induced tumors. (C) Mean aggregate tumor weight in mice carrying control or mP2Y12 CRISPR-Cas9 ID8-VEGF cell–induced tumors (n = 10 per group). (D-E) Effect of ecto-apyrase (CD39) gene knockdown using hCD39 siRNA on the platelet-induced increase in the proliferation rate in A2780ip2 (D) and SKOV3ip1 (E) human ovarian cancer cells (n = 3 triplicate experiments). (F) Expression of CD39 in tumor nodules induced by WT A2780ip2 or A2780ip2-CD39 CRISPR-Cas9 cells. (G) Mean tumor weight aggregate in mice carrying tumors induced by WT or A2780ip2-CD39 CRISPR-Cas9 cells (n = 6 mice per group).

Effect of P2Y12 gene knockdown in murine ovarian cancer cells on the growth of orthotopic ovarian tumors. (A) Expression of P2Y12 mRNA in control and mP2Y12 CRISPR-Cas9 ID8-VEGF cells before the injection into mice (n = 3 triplicate experiments). (B) Representative images of necropsy in mice carrying control or mP2Y12 CRISPR-Cas9 ID8-VEGF cell–induced tumors. (C) Mean aggregate tumor weight in mice carrying control or mP2Y12 CRISPR-Cas9 ID8-VEGF cell–induced tumors (n = 10 per group). (D-E) Effect of ecto-apyrase (CD39) gene knockdown using hCD39 siRNA on the platelet-induced increase in the proliferation rate in A2780ip2 (D) and SKOV3ip1 (E) human ovarian cancer cells (n = 3 triplicate experiments). (F) Expression of CD39 in tumor nodules induced by WT A2780ip2 or A2780ip2-CD39 CRISPR-Cas9 cells. (G) Mean tumor weight aggregate in mice carrying tumors induced by WT or A2780ip2-CD39 CRISPR-Cas9 cells (n = 6 mice per group).

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