American Society of Hematology

Figure 4.

$Figure 4. G-CSF-induced sympathetic tone provokes fever via PGE2 production by neutrophils. (A) Dot-plot profile of G-CSF (4 doses)-primed BM CD11b+ cells. (B) PGE2 levels in culture supernatants of each fraction (Gr-1high neutrophils: CD11b+F4/80dimGr-1high, Gr-1low neutrophils: CD11b+F4/80dimGr-1low, monocytes/macrophages: CD11b+F4/80highGr-1low; 4-hour incubation with no stimulation; n = 3). (C) Body temperature during G-CSF treatment with or without neutrophil depletion by anti-Gr-1 antibody (1A8; n = 5). (D) Protocol for chemical denervation by 6OHDA followed by 4 doses of G-CSF. (E) PGE2 levels in culture supernatants of sorted BM neutrophils after 4 doses of G-CSF with or without chemical denervation by 6OHDA. Vehicle: 0.1% ascorbic acid (n = 7). (F) Body temperature during G-CSF treatment with or without chemical denervation by 6OHDA (n = 7). Data are represented as mean ± SEM. *P < .05; **P < .01; ***P < .001. n.s., not significant.$

G-CSF-induced sympathetic tone provokes fever via PGE₂production by neutrophils. (A) Dot-plot profile of G-CSF (4 doses)-primed BM CD11b⁺ cells. (B) PGE₂ levels in culture supernatants of each fraction (Gr-1^high neutrophils: CD11b⁺F4/80^dimGr-1^high, Gr-1^low neutrophils: CD11b⁺F4/80^dimGr-1^low, monocytes/macrophages: CD11b⁺F4/80^highGr-1^low; 4-hour incubation with no stimulation; n = 3). (C) Body temperature during G-CSF treatment with or without neutrophil depletion by anti-Gr-1 antibody (1A8; n = 5). (D) Protocol for chemical denervation by 6OHDA followed by 4 doses of G-CSF. (E) PGE₂ levels in culture supernatants of sorted BM neutrophils after 4 doses of G-CSF with or without chemical denervation by 6OHDA. Vehicle: 0.1% ascorbic acid (n = 7). (F) Body temperature during G-CSF treatment with or without chemical denervation by 6OHDA (n = 7). Data are represented as mean ± SEM. *P < .05; **P < .01; ***P < .001. n.s., not significant.

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