Neutrophil precursor cell line produces PGE₂by β-adrenergic stimulation. (A) PGE₂ levels in culture supernatants of hematopoietic cell lines (8 hours after stimulation with indicated reagents; n = 3-4). Vehicle: PBS. G, G-CSF; Iso, isoproterenol. (B) qRT-PCR analysis of mPGES-1 mRNA in cell lines in A (n = 3). (C, D) PGE₂ levels in culture supernatants of 32D cells (8 hours after stimulation with indicated reagents; n = 3-4). NE, norepinephrine; Indo, indomethacin. (E) Schema of deuterium-labeled arachidonic acid (AA-d₁₁) assay. (F) Deuterium-PGE₂ (PGE₂-d₁₁) levels in 32D culture (n = 14). Data are represented as mean ± SEM. *P < .05; **P < .01; ***P < .001.