mPGES-1-mediated PGE₂production from hematopoietic cells modulates G-CSF-induced fever and mobilization. (A) Pathway of PGE₂ synthesis. (B) Body temperature in mPGES-1^+/+ and mPGES-1^−/− mice during G-CSF treatment (n = 4-6). The x axes show the G-CSF dose (every 12 hours) and the timing to measure the body temperature after each dose of G-CSF. (C) CFU-Cs mobilized in mPGES-1^+/+ and mPGES-1^−/− mice (n = 8-10 for PBS/BSA group and n = 26-28 for G-CSF group). (D) qRT-PCR analysis of mPGES-1 mRNA in BM cells during G-CSF treatment (n = 9-10 per group). (E) PGE2 levels assessed by LC-MS/MS in whole BM (BM cells and extracellular fluids) at 2 hours after 4 doses of G-CSF (n = 8). (F) CFU-Cs mobilized in mPGES-1 chimeric mice (n = 3-5 for PBS/BSA group and n = 6 for G-CSF group). Data are represented as mean ± SEM. *P < .05; **P < .01.