American Society of Hematology

Figure 5.

$Figure 5. hESCs differentiated in the presence of SR-1 skews development toward cNK cells whereas TCDD supports the development of an ILC phenotype. (A) Representative flow cytometry profile of nonadherent hematopoietic cells differentiated from hESCs in the presence of DMSO, SR-1, or TCDD at day 11+28. (B) cNK, NKP, and NKP/ILC subpopulations from day 11+28 DMSO-, SR-1–, and TCDD-differentiated hESCs assessed for CD56 and LFA (CD11a/CD18) surface antigen expression. Representative flow cytometry plots are shown; n = 3. (C) Total percentage of cNK, NKP, and NKP/ILCs present in the nonadherent fraction of differentiating hESCs in the presence of DMSO, SR-1, or TCDD at day 11+28; n = 3, error bars represent SEM. *P < .05, **P < .01, ***P < .001 as compared with DMSO-treated controls and assessed by 2-way ANOVA + Tukey-Kramer multiple comparisons post hoc test. (D) CD94−CD117+ subpopulations were further quantified for expression of LFA+ (NKP) and LFA− (ILC) by flow cytometry; n = 3, error bars represent SEM. *P < .05, **P < .01 as compared with DMSO-treated controls and assessed by 2-way ANOVA + the Tukey-Kramer multiple comparisons post hoc test.$

hESCs differentiated in the presence of SR-1 skews development toward cNK cells whereas TCDD supports the development of an ILC phenotype. (A) Representative flow cytometry profile of nonadherent hematopoietic cells differentiated from hESCs in the presence of DMSO, SR-1, or TCDD at day 11+28. (B) cNK, NKP, and NKP/ILC subpopulations from day 11+28 DMSO-, SR-1–, and TCDD-differentiated hESCs assessed for CD56 and LFA (CD11a/CD18) surface antigen expression. Representative flow cytometry plots are shown; n = 3. (C) Total percentage of cNK, NKP, and NKP/ILCs present in the nonadherent fraction of differentiating hESCs in the presence of DMSO, SR-1, or TCDD at day 11+28; n = 3, error bars represent SEM. *P < .05, **P < .01, ***P < .001 as compared with DMSO-treated controls and assessed by 2-way ANOVA + Tukey-Kramer multiple comparisons post hoc test. (D) CD94⁻CD117⁺ subpopulations were further quantified for expression of LFA⁺ (NKP) and LFA⁻ (ILC) by flow cytometry; n = 3, error bars represent SEM. *P < .05, **P < .01 as compared with DMSO-treated controls and assessed by 2-way ANOVA + the Tukey-Kramer multiple comparisons post hoc test.

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