American Society of Hematology

Figure 3.

Src kinase, NOX, and O2•−/H2O2 are important for platelet ERK5 activation by oxLDL-CD36 signaling. ERK5 activation by 15-minute stimulation with oxLDL was detected in human washed platelets (3 × 108/mL) after the pretreatment of (A) control Src inhibitor PP3 and Src inhibitor PP2 (10 µM) (n = 4), (B) NOX peptide inhibitor gp91ds-Tat or control scrambled-Tat (2 µM) (n = 3), (C) nitric oxide donor DPTA-NONOate (10 µM) to scavenge O2•− (n = 3), and (D) PEG-catalase (1000 U/mL) (n = 5). Data represented as mean ± SEM. P value determined by 1-way ANOVA with Tukey post hoc analysis.

Src kinase, NOX, and O₂^•−/H₂O₂ are important for platelet ERK5 activation by oxLDL-CD36 signaling. ERK5 activation by 15-minute stimulation with oxLDL was detected in human washed platelets (3 × 10⁸/mL) after the pretreatment of (A) control Src inhibitor PP3 and Src inhibitor PP2 (10 µM) (n = 4), (B) NOX peptide inhibitor gp91ds-Tat or control scrambled-Tat (2 µM) (n = 3), (C) nitric oxide donor DPTA-NONOate (10 µM) to scavenge O₂^•− (n = 3), and (D) PEG-catalase (1000 U/mL) (n = 5). Data represented as mean ± SEM. P value determined by 1-way ANOVA with Tukey post hoc analysis.

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