Asymmetric distribution of patient-derived LSC clones in murine xenografts. At euthanization of the mice, leukemia cells from patient-derived xenografts were isolated from 7 anatomic locations; barcode composition was determined by polymerase chain reaction and deep sequencing. (A) Schematic representation of the skeletal locations analyzed. (B,C) Anatomic distribution of barcoded clones in 2 representative xenografts of ALL-58.1. (Top) Relative barcode abundance per location. Each color is 1 barcode clone. (Bottom) Similarity in clonal distribution, assessed by Euclidean distance (for detailed information on methods, see supplemental File 8). (D-E) Relative clonal abundance per anatomic location. Clones are ranked based on their overall abundance (x-axis). The y-axis shows the relative abundance of each clone to the total pool in 1 location. Dots on the same vertical line reflect the size of a particular clone in different anatomic locations. The mean contribution of each clone is shown with a horizontal line. (F-H) Clonal diversity related to anatomical location (F); transplanted cell dose (G) or serial transplantation (H). Depicted are Euclidean distances over all combinations of anatomic locations within individual mice. Different colors are used to depict individual mice.