Clonal complexity of patient-derived leukemia subclones. (A-C) Clonal composition in blood of primary xenografts of patient-derived leukemia. Each bar represents 1 time point; each colored rectangle 1 barcode. The height of the bars corresponds to the %hCD45+ cells, determined by flow cytometry. (D-F) Clonal composition in BM of primary xenografts at euthanization. Each bar represents spine BM of 1 mouse. For ALL-70 (F), the mouse transplanted with 1.4 × 104 cells is marked with an asterisk. (G) Barcode complexity in blood and BM of primary patient-derived xenografts over time. (H) Plot of retrieved barcode complexity in primary xenografts vs transplanted cell dose. Each dot represents 1 sample (ie, 1 anatomic location). (I) Plot of LPC frequency in primary xenografts vs transplanted cell dose. LPC frequency was calculated by dividing the number of retrieved clones by the administered cell dose.
