Figure 3.
Figure 3. Increased apoptosis of splenic donor NK cells during the early phase of GVHD. (A) Donor NK cells in the BM (top) and spleen (bottom) at day 7 and day 14 posttransplant (B6→B6D2F1) were stained with Sytox Blue and Annexin V and analyzed by flow cytometry to quantify live cells (live/dead−Annexin V−), apoptotic cells (live/dead−Annexin V+), and dead/necrotic cells (live/dead+Annexin V+). Representative dot plots from the BM and spleen at the time points indicated are shown. Day 7 mean ± SEM pooled from 2 independent experiments per time point; n = 7-8 per group. Day 14 mean ± SD from 1 experiment; n = 4 per group. (B) Ki-67 expression by donor NK cells in the BM and spleen (B6→B6D2F1). Representative histograms shown with the same isotype control (shaded histogram) shown for day 7 and day 14. Mean ± SD from 1 experiment; n = 4-6 per group per time point.

Increased apoptosis of splenic donor NK cells during the early phase of GVHD. (A) Donor NK cells in the BM (top) and spleen (bottom) at day 7 and day 14 posttransplant (B6→B6D2F1) were stained with Sytox Blue and Annexin V and analyzed by flow cytometry to quantify live cells (live/dead−Annexin V−), apoptotic cells (live/dead−Annexin V+), and dead/necrotic cells (live/dead+Annexin V+). Representative dot plots from the BM and spleen at the time points indicated are shown. Day 7 mean ± SEM pooled from 2 independent experiments per time point; n = 7-8 per group. Day 14 mean ± SD from 1 experiment; n = 4 per group. (B) Ki-67 expression by donor NK cells in the BM and spleen (B6→B6D2F1). Representative histograms shown with the same isotype control (shaded histogram) shown for day 7 and day 14. Mean ± SD from 1 experiment; n = 4-6 per group per time point.

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