NK-cell reconstitution is impaired in GVHD. (A) B6 (non-GVHD) and B6D2F1 (GVHD, MHC-mismatch) mice were transplanted with B6.CD45.1 BM and CD3⁺ T cells. Donor-derived CD3⁻CD19⁻NKp46⁺CD122⁺ NK cells were enumerated thereafter. Representative dot plots from the spleen at the time points indicated are shown. Mean ± standard error of the mean (SEM) pooled from 2 or 3 independent experiments per time point for BM, spleen, pLNs. Data shows mean ± standard deviation (SD) from a single experiment per time point for blood, liver, and lung; n = 4-14 per group per time point. Representative plots of NKp46 and NK1.1 staining in the BM and spleen at day 14 posttransplant. (B) B6 (non-GVHD) and BALB/b (GVHD, MHC-matched, minor histocompatibility antigen-mismatched) mice were transplanted with B6.CD45.1 BM and CD3⁺ T cells and donor NK cells enumerated thereafter. Representative dot plots from the spleen at the time points indicated are shown. Mean ± SEM pooled from 1 or 2 independent experiments per time point for all tissues; n = 3-8 per group per time point. Tx, transplant.