In vivo iPlt generation is decreased for FLI1-low and increased for FLI1-high lines. (A,B) NSG mice were infused with iMegs, and percentage of human platelets was determined at various points up to 24 hours. Means ± 1 SEM are shown with 4-7 independent experiments per arm. (A, left) Data analyzed per infused iMegs generated from isogenic genome-edited iPSC lines. (Right) Data analyzed per infused iMegs WT iPSCs and the 2 PTSx lines. (B) Same as in panel A, but analyzed per initial HPCs from which the iMegs were prepared. (C-D) Area under the curve (AUC) calculations for iPlt generation either from iMegs (C) or from HPCs (D). Means ± 1 SEM are shown with number of independent experiments per arm shown in each bar. Significant P values were determined using 1-way ANOVA.