Effect of absence of p53 on intrathrombotic cytokine expression. (A) Intrathrombotic expression of IL-6 mRNA at day 8 and day 12 after vena cava ligation was determined by qPCR. Values represent the mean ± SEM (n = 3 Tp53^+/+ and 4 Tp53^−/−). *P < .05, Tp53^+/+ vs Tp53^−/−. (B) Intrathrombotic expression of IL-6 protein at day 12 was determined by enzyme-linked immunosorbent assay (ELISA). All values represent the mean ± SEM (n = 4 Tp53^+/+ and 5 Tp53^−/−). **P < .02, Tp53^+/+ vs Tp53^−/−. (C) Enumeration of intrathrombotic macrophages at day 12 after vena cava ligation by immunohistochemical staining using anti-F4/80 antibody; original magnification ×200. Scale bar, 200 μm. Representative images from 4 independent animals are shown. (D) The number of macrophages was quantified as described in “Methods.” All values represent the mean ± SEM (n = 4 Tp53^+/+ and 4 Tp53^−/−). (E) Intrathrombotic gene expression of macrophage polarization markers at 8 days (A) and 12 days (B) after vena cava ligation in Tp53^+/+ and Tp53^−/− animals were determined by qPCR. All values represent the mean ± SEM (n = 3 Tp53^+/+ and 4 Tp53^−/−). *P < .05, Tp53^+/+ vs Tp53^−/−. hpf, high-powered field; iNOS, inducible NO synthase.