Characterization of venous thrombi from Tp53^+/+and Tp53^−/−mice at 4 days after vena cava ligation. Immunohistochemical analysis of intrathrombotic neutrophil accumulation was performed using anti-Lys6G antibodies (A) and macrophage accumulation was analyzed using anti-F4/80 antibodies (B) in venous thrombus samples from Tp53^+/+ and Tp53^−/− mice. (C) Collagen content in resolving Tp53^+/+ and Tp53^−/− thrombi at day 4 was determined in histological sections after Picrosirius Red staining; original magnification ×200. Scale bar, 200 μm. Representative results from 4 to 5 independent animals are shown. The numbers of Lys6G⁺ cells (neutrophils) (D) and F4/80⁺ cells (macrophages) (E) were determined as described in “Methods.” All values represent the mean ± standard error of the mean (SEM) (n = 4 Tp53^+/+ and 5 Tp53^−/−).