Profile of macrophages recruited to the pleural cavity after injection of Plg/Pla. BALB/c mice were challenged by an i.pl. injection of Plg (2 µg per cavity), Pla (2 µg per cavity), or PBS (vehicle). The cells obtained from the pleural lavage 48 hours after injection of Plg/Pla were analyzed by flow cytometry according to their size and granularity and expression of surface molecules F4/80, CD11b, and Gr1, as described in the gating strategy (A). The cells that migrated to the cavity were classified as M1 (F4/80^low Gr1⁺ Cd11b^med) (B), M2 (F4/80^high Gr1^– CD11b^high) (C), and Mres (F4/80^med CD11b^low) (D) subpopulations of macrophages. Results are expressed as the number of cells and as the mean ± SEM of at least 4 mice in each group. The experiment was performed 3 times with similar results. *P < .05; **P < .01 when compared with mice challenged with PBS.