Unsupervised hierarchical clustering of combined ADP and ADP+Iloprost temporal profiles. Color codes reflect log2 ratios compared with unstimulated controls, square sizes indicate relative standard deviations (RSD, see supplemental Methods). Entries are given as: Gene name, phosphorylation site, protein description. (A) Complete node map. All temporal phosphorylation profiles (central gray node) were clustered into different communities according to their similarities, out of which certain examples were selected that indicate functional nodes. (B) Node representing phosphopeptides with early and sustained upregulation, 5 of which are potential PKC targets.²⁶  (C) Node with phosphopeptides not or only slightly affected by ADP but upregulated by Iloprost treatment, consequently potential PKA targets.²⁶  (D) Node with phosphopeptides upregulated by ADP and downregulated by subsequent Iloprost treatment.