Hbb^th3/+ neutrophils display reduced chemotaxis. Transwell migration assays were used to assess neutrophil chemotaxis. Percentage of neutrophils (CD49b^negCD11b⁺Ly6G^highLy6C^low) migrating toward each indicated concentration of CXCL2 is shown for (A) BM, (B) blood, and (C) spleen. Representative histograms of fluorescence intensity of BM, blood, and spleen neutrophils (CD49b⁻CD11b⁺Ly6G^highLy6C^low) following antibody staining for (D-F) CXCR2 and (G-I) CXCR4. Representative histogram for isotype control antibody staining is shown in yellow; WT cells in blue, and Hbb^th3/+ cells in red. Mean median fluorescence intensity (MFI) values in BM, blood (BL), and spleen (SP) neutrophils in WT and Hbb^th3/+ mice following antibody staining for (J) CXCR2 and (K) CXCR4. Data are presented as mean ± SEM, n ≥ 3 per mice group. Results representative of 3 independent experiments are shown. Statistical significance was evaluated with Student t test.