Analysis of erythroid cells from patients with a mutation in GATA1. (A) Flow cytometric analysis of CD34⁺ cells from the carrier mother and a DBA patient with a GATA1 mutation cultured for 14 days. The cells were labeled with CD235, CD44, and CD41 antibodies. (B) Lysates from differentiating erythroid cells from a patient with a GATA1 mutation, the carrier mother, and a control donor were immunoblotted with antibodies against GATA1 and α-tubulin (loading control).